Fig. 8. EGFR is down-regulated by DZNep, and its inhibition reduces cell survival in chondrosarcomas. (A) EGFR protein expression in chondrosarcomas treated with 1 µM DZNep for 72 h were analyzed by Western blot. β-actin was a loading control. (B) Chondrosarcomas were treated with gefitinib, an EGFR inhibitor, for 72 h. Then cell survival was assayed using Cell Titer-Glo luminescent cell viability assay kit. Histograms represent means + SEM (n=3). *: p-value < 0.05; **: p-value < 0.01; ***: p-value < 0,001.